Enhanced expression of recombinant miraculin, a tasting modifying protein, in Nicotiana tabacum hairy roots using 18.2 heat shock protein promoter and terminator

Miraculin, a taste modifier, is protein that was first isolated from miracle fruit (Richadella dulcifica). It can change sour into sweet when acids are consumed, although it does not elicit response. Miraculin may have the potential in industry as substitute for sugars and artificial sweeteners. Since plant has low productivity, mass production of miraculin limited. Transgenic hairy root culture alternative system miraculin. In this study, we investigated expression recombinant tobacco (Nicotiana tabacum) roots. To increase expression, heat shock 18.2 promoter terminator were used to drive gene host system. Synthetic transformed Nicotiana tobacum leaf explants via Agrobacterium rhizogenes. The transgenic clones contained synthetic showed rapid growth reached maximum after 35-day culture. When regulated by terminator, increased than control CaMV 35S nopaline synthase terminator. 19.97 ng per µg total soluble equivalently with approximately 2% protein. For time, modifying successfully expressed root. results study given promising approach application produce